■ 基本信息
啟動子: | URA3，T7,T3 |
復制子: | 2μFI |
質(zhì)粒分類: | 酵母系列，釀酒酵母表達載體 |
質(zhì)粒大小: | 5726bp |
原核抗性: | Amp |
真核抗性: | URA3 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 酵母細胞 |
誘導方式: | 半乳糖誘導 |
5'測序引物: | M13R:CAGGAAACAGCTATGACC |
3'測序引物: | 根據(jù)序列設計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 酵母菌 |
質(zhì)粒用途: | 蛋白表達 |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: | URA3
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熒光標記: |
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■ 質(zhì)粒簡介
The order of the major features in this plasmid is:ori(f1) - lacZ - T7 promoter - MCS (KpnI-SacI) - T3 promoter - lacI - ori(pMB1) - ampR - ori (2 micron) - URA3. Contains the REP3 and FRT sequences necessary for high copy propagation in yeast. In S. cerevisiae, the copy number is about 20 per haploid cell. In non-selective growth, plasmids are lost through mitotic segregation at rates in the range of 4.4 + or - 1.4% of progeny per doubling. YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing. Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; PstI--4.0, 1.7; PvuII--5.2, 0.54. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 416322 Hosts: E.coli DH5alpha, Saccharomyces cerevisiae, E.coli.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK963pRS426釀酒酵母質(zhì)粒.txt
