■ 基本信息
啟動(dòng)子: | Ac5 |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類: | 昆蟲系列質(zhì)粒；昆蟲游離質(zhì)粒；昆蟲胞內(nèi)質(zhì)粒 |
質(zhì)粒大小: | 6118bp |
質(zhì)粒標(biāo)簽: | N-EGFP，C-V5，C-6×His |
原核抗性: | Amp |
真核抗性: | 無(wú) |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 果蠅細(xì)胞系S2 |
誘導(dǎo)方式: | 無(wú)需誘導(dǎo)，組成型表達(dá) |
5'測(cè)序引物: | Ac5-F(ACACAAAGCCGCTCCATCAG) |
3'測(cè)序引物:
| BGH-R(TAGAAGGCACAGTCGAGG)
|
備注: | 非病毒 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 昆蟲細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
|
熒光標(biāo)記: | 綠色
|
■ 質(zhì)粒簡(jiǎn)介
pAC5.1b-EGFP-2質(zhì)粒是一個(gè)昆蟲細(xì)胞組成型表達(dá)載體，Ac5啟動(dòng)子驅(qū)動(dòng)EGFP標(biāo)簽、目的基因、Myc標(biāo)簽和6×HIS標(biāo)簽一起融合表達(dá)，可以與篩選載體pCoHygro或者pCoBlast共轉(zhuǎn)染，建立穩(wěn)表達(dá)細(xì)胞系。
pAC5.1b-EGFP② is a 6kb expression vector designed for use with the Drosophila Constitutive Expression System available from Invitrogen. Upon transfection, the vector allows transient expression of your protein of interest in Drosophila cells. When cotransfected with the selection vector, pCoHygro or pCoBlast, pAC5.1b-EGFP allows selection of stable cell lines exhibiting constitutive expression of the protein of interest.
? The Drosophila actin 5C (Ac5) promoter for high-level, constitutive expression of the gene of interest in S2 cells (Chung and Keller, 1990).
? Multiple cloning site to facilitate cloning the gene of interest.
? C-terminal peptide containing the V5 epitope and polyhistidine (6xHis) tag for detection and purification of your protein of interest (if desired).
? Three reading frames to facilitate in-frame cloning with the C-terminal peptide.
? N-terminal EGFP tag for better detection.
? Ampicillin resistance gene for selection of transformants in E. coli.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK1035pAC5.1b-EGFP-2昆蟲胞內(nèi)質(zhì)粒.txt
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
